OpenCV-Based Nanomanipulation Information Extraction and the Probe Operation in SEM

Fuzzy Control and Connected Region Marking Algorithm-Based SEM Nanomanipulation

The interactive nanomanipulation platform is established based on fuzzy control and connected region marking CRM algorithm in SEM. The 3D virtual nanomanipulation model is developed to make up the insufficiency of the 2D SEM image information, which provides the operator with depth and real-time visual feedback information to guide the manipulation. The haptic device Omega3 is used as the maste...

In Situ SEM Nanomanipulation and Nanomechanical/Electrical Characterization

Nanomanipulation of Small Objects in a Feg-sem

Nanometric objects attract huge interest due to their new and unexpected physical and chemical properties [1], which may allow the development of new technological devices. The study of these nano-objects is seriously limited by the fact that well-established conventional tools can not be easily applied in this size range. The development of new instruments and methodologies is necessary to all...

Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures

Nanomanipulation under scanning electron microscopy (SEM) enables direct interactions of a tool with a sample. We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus. In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescenc...

Performing probe experiments in the SEM.

A four nanoprobe system has been installed inside a FEI XL30 F scanning electron microscope (SEM), and shown to be fully compatible with the normal functions of the SEM and also a Gatan cold stage (model C1003, -185-400 degrees C). With some selected examples of applications, we have shown that this nanoprobe system may be used effectively for gripping, moving and manipulating nanoobjects, e.g....